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KMID : 0357119950170010001
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Korean Journal of Immunology
1995 Volume.17 No. 1 p.1 ~ p.10
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Effect of in vitro High Temperature on Antiviral Function of Interferon and 2' -5' Oligoadenylate Synthetase Activity
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Abstract
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In this report we performed plaque reduction test using Hep-2 cell and vesiculo-stomatitis virus(VSV) at 37℃and 39℃examine the effect of high temperature on the antiviral activity of human IFN-γ. And we measured the activity of 2-5A synthetase
to
determine the effect of high temperature on the 2-5A system of Hep-2 cells treated with IFN-α or IFN-γ at 39℃and at 37℃.
In the case of the treatment of Hep-2 cells with IFN-α or IFN-γ before VSV inoculation, the antiviral actions of IFN-α and IFN-γwere greater at 39℃than at 37℃. In the case of INF-αor IFN-γ treatment after VSV inoculation, no antiviral
action of
IFN-α or IFN-γ was boserved. Enhancement of 2-5A synthetase activities by IFN-α or IFN-γ were greater at 39℃ than at 37℃.
From this study, it may be concluded that the antiviral action of IFN-γand the activity of the 2-5A system are enhanced by high temperature. It seems that enhancement of the 2-5A system activity at high temperature may contribute to the
enhancement of
antiviral action of INF-γ.
It is reported that different characters in 2-5A system activation by IFN-αor IFN-γ. Thus the mechanisms of enhancement of 2-5A system activation by IFN-γat high temperature should be further studyied.
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